Morphological and virological studies in six autopsies of children with adenovirus pneumonia

Pulmonary lesions compatible with adenovirus infection were detected by gross and microscopic examination of autopsy tissues children aged from 5 to 34 months. Hepatic lesions indicative of systemic infection were also found in four of the chisldren. The viral etiology was confirmed in three cases by in-situ hibridization, electro-microscopy and immunofluorescence performed in parafin-embedded tissues, and in one case by cell culture isolation of adenovirus type 2 from nasopharyngeal exudate. Routine testing by methods additional to conventional light microscopy would probably have revealed a larger number of adenovirus infections among the 1.103 autopsy records analyzed in this study

Comparison of the effect of calcium withdrawal from the medium and of blockade of extracellular calcium entry by isradipine on the contractile responses of the isolated rat stomach

The role of calcium in drug-induced contractions of rat gastric fundus strips was evaluated by determining the effect of two procedures on the dose-respponse curves of agonists: a) removal of calcium from the nutrient solution and b) blockade of calcium channels with the dihydrophydine isradipine. Gastric strips were obtanied from adult Wistar rats and suspended in Tyrode solution at 37-C for contraction studies. Dose-response curves for carbachol (CCh), serotonin (5-HT), KCl and BaCl2 were constructed under the two conditions descrived above. A complete blockade of contractile effects was observed for 5-HT and KCl 60 min after calcium withdrawal of after using 3 mM (45 min) of the calcium antagonist. A lower dose of antagonist or a shorter incubation in calcium-free solution caused a partial decrease of dose-response curves, added to a 30-fold shift to the right after the calcium antagonist (1mM), or a larger than 100-fold shift 3 min after calcium removal. In contrast, dose-response curves for CCh and BaCl2 were not significantly affected by either type of treatment. It is concluded that 5-HT and KCl utilize extracellular sources of calcium, whereas CCh or BaCl2 depends on a tightly-bound calcium pool in this preparation

Infeccoes pre-natais. Avaliacao de metodo simplificado (Rapi Tex IgM) em sua triagem. / Prenatal infections.Evaluation of a simplified method (Rapi Tex IgM) to their selection

Os autores submeteram 500 recem-nascidos a teste simplificado (Rapi Tex IgM) que por simples reacao de aglutinacao evidencia quantidades anormalmente elevadas de IgM no sangue do cordao umbilical. Os resultados obtidos pela triagem foram avaliados atraves de dosagens de IgM no sangue do cordao umbilical exame morfologico de placentas (macro e microscopia), acompanhamento clinico e laboratorial de alguns casos e exame anatomopatologico dos casos em que ocorreu o obito. Concluem pela validade do teste na triagem de infeccoes pre-natais

Causas infecciosas das anomalias congenitas. / Infective causes of congenital anomalies

Revisao sumaria das causas infecciosas das anomalias congenitas englobando as malformacoes, que correspondem a estruturas anormais organicas ou tissulares decorrentes de erros primarios de morfogenese embrionaria e deformacoes, que se instalam no periodo fetal da vida intrauterina, correspondentes a alteracoes de forma e estrutura de orgaos primitivamente bem constituidos